基于放屁虫的生物传感器。

Bombardier beetle based biosensor.

作者信息

Nakatani H S, de Oliveira Neto G, Godinho O E, Aleixo L M, Zinner K

机构信息

Instituto de Química, Universidade Estadual de Campinas, Brazil.

出版信息

Biosens Bioelectron. 1996;11(5):489-92. doi: 10.1016/0956-5663(96)86785-6.

DOI:10.1016/0956-5663(96)86785-6

PMID:8729239

Abstract

A biosensor constructed by combining an oxygen electrode with the liquid ejected by bombardier beetles (Pheropsophus aequinoctialis) immobolized on a collagen membrane is developed for hydrogen peroxide. The sensor presents a linear range of 2.0 x 10(-4)-2.0 x 10(-3) M and a reproductibility of approximately 2%. It shows a better lifetime than similar ones employing purified catalase. The selectivity is good since other enzymes are not present in the natural source employed.

摘要

一种通过将氧电极与固定在胶原膜上的射炮步甲（Pheropsophus aequinoctialis）喷出的液体相结合构建的生物传感器被开发用于检测过氧化氢。该传感器的线性范围为2.0×10⁻⁴ - 2.0×10⁻³ M，重现性约为2%。与使用纯化过氧化氢酶的类似传感器相比，它具有更长的使用寿命。由于所使用的天然来源中不存在其他酶，因此选择性良好。