de Paiva M L, Contel E P
Departmento de Genética e Matemática Aplicada à Biologia, Brasil.
Rev Bras Biol. 1995 Dec;55 Suppl 1:107-11.
Hexokinase enzyme was studied by means of starch-agarose gel electrophoresis in Plebeia droryana. Three anodal bands with enzyme activity were observed during the development: hexokinase-1 (HK-1), the intensity of which increases from larvae to adult, probably related with energy supply to thoracic flight muscles and, therefore, with flight activity; hexokinase-2 (HK-2), that reaches maximum intensity in clear brown eyed pupae and hexokinase-3 (HK-3), the intensity of which reaches maximum peak in winged pupae and is not observed in the adult phase. This isozyme should have important functions in the bee metamorphosis.
通过淀粉 - 琼脂糖凝胶电泳对德鲁氏无刺蜂(Plebeia droryana)中的己糖激酶进行了研究。在发育过程中观察到三条具有酶活性的阳极带:己糖激酶 -1(HK -1),其强度从幼虫到成虫逐渐增加,可能与胸部飞行肌肉的能量供应有关,因此与飞行活动有关;己糖激酶 -2(HK -2),在浅褐色眼蛹中达到最大强度;以及己糖激酶 -3(HK -3),其强度在有翅蛹中达到最高峰,在成虫阶段未观察到。这种同工酶在蜜蜂变态过程中应该具有重要功能。