通过原位杂交检测甲状腺乳头状癌中促甲状腺激素受体信使核糖核酸（TSH-R mRNA）的异质性分布。

Heterogeneous distribution of thyrotrophin receptor messenger ribonucleic acid (TSH-R mRNA) in papillary thyroid carcinomas detected by in situ hybridization.

作者信息

Tanaka K, Inoue H, Miki H, Komaki K, Monden Y

机构信息

Second Department of Surgery, University of Tokushima, Japan.

出版信息

Clin Endocrinol (Oxf). 1996 Mar;44(3):259-67.

PMID:8729520

Abstract

OBJECTIVE

TSH suppression therapy has been reported to be effective in some cases of papillary carcinoma of the thyroid. However, there has been little information regarding the status of expression of TSH-R mRNA in papillary carcinomas. Therefore, we examined the expression of TSH receptor (TSH-R) mRNA in thyroid tumours by in situ hybridization.

DESIGN

Surgically resected thyroid tumour specimens were used. The expression level of TSH-R mRNA was detected by in situ hybridization, and the difference between tumours and their adjacent normal thyroid tissues was assessed.

PATIENTS

Tumours and adjacent normal thyroid tissues were obtained from nine patients with differentiated papillary carcinomas and eight with adenomas.

MEASUREMENTS

We performed in situ hybridization on frozen sections of thyroid tissues. Digoxigenin-labelled oligonucleotide was used to hybridize and to detect TSH-R mRNA. We counted positively stained cells and non-stained cells in each section, calculated the positivity of stained cells and assessed the distribution of positively stained cells.

RESULTS

In normal thyroid tissue, the positivity was 95.3 +/- 2.3% (mean +/- SD) and the distribution of TSH-R mRNA was homogeneous. In adenoma, positivity was 93.5 +/- 4.4%. In most cases of adenoma, the distribution was homogeneous. In papillary carcinoma, the expression level of TSH-R mRNA was significantly lower and the positivity was 81.9 +/- 12.6%. Furthermore, in five cases of papillary carcinoma, the distribution was heterogeneous; four of these cases were stage 3, while the remaining case was stage 1. Three of the four cases with a homogeneous distribution were stage 1, while one case was stage 3.

CONCLUSIONS

The heterogeneity of TSH-R mRNA distribution suggests the effect of TSH on carcinoma cells may be variable. Furthermore, papillary carcinomas of advanced clinical stage showed a tendency to low expression and heterogeneous distribution of TSH-R mRNA.

摘要

目的

据报道，促甲状腺激素（TSH）抑制疗法在某些甲状腺乳头状癌病例中有效。然而，关于甲状腺乳头状癌中TSH-R mRNA的表达状况，相关信息较少。因此，我们通过原位杂交检测了甲状腺肿瘤中促甲状腺激素受体（TSH-R）mRNA的表达。

设计

使用手术切除的甲状腺肿瘤标本。通过原位杂交检测TSH-R mRNA的表达水平，并评估肿瘤与其相邻正常甲状腺组织之间的差异。

患者

从9例分化型乳头状癌患者和8例腺瘤患者中获取肿瘤及相邻正常甲状腺组织。

测量

我们对甲状腺组织的冰冻切片进行原位杂交。使用地高辛标记的寡核苷酸进行杂交并检测TSH-R mRNA。我们对每个切片中阳性染色细胞和未染色细胞进行计数，计算染色细胞的阳性率，并评估阳性染色细胞的分布。

结果

在正常甲状腺组织中，阳性率为95.3±2.3%（平均值±标准差），TSH-R mRNA的分布均匀。在腺瘤中，阳性率为93.5±4.4%。在大多数腺瘤病例中，分布均匀。在乳头状癌中，TSH-R mRNA的表达水平显著降低，阳性率为81.9±12.6%。此外，在5例乳头状癌中，分布不均匀；其中4例为3期，其余1例为1期。4例分布均匀的病例中有3例为1期，1例为3期。