Matsuda S, Baluk P, Shimizu D, Fujiwara T
Department of Anatomy, Ehime University School of Medicine, Japan,
Anat Embryol (Berl). 1996 May;193(5):475-80. doi: 10.1007/BF00185878.
The aim of this study was to compare the morphological development of dorsal root ganglion neurons in embryonic and early postnatal chicks and rats. The three-dimensional architecture of neurons was observed in ganglia in situ and in dissociated neurons by scanning electron microscopy after removal of the capsule and connective tissue. The percentages of neurons at different developmental stages were determined. The general morphological changes in the chick resembled those in the rat but the timing was different. In both chick and rat, the majority of neurons were bipolar at early stages of development (embryonic day 6 in chick and day 14 in rats) and later underwent pseudo-unipolarization to become mostly unipolar neurons at hatching or birth. This maturation event started at an earlier stage in chick embryos than in rats, with 57% unipolar neurons in chick and only 7% in rat on embryonic day 14. However, just after hatching or birth, at day 22 of development, a larger proportion of immature unipolar neurons remained in chicks (13%) than in rats (3%). We conclude that these differences should be taken into consideration in designing experiments on dorsal root ganglion neurons grown in tissue culture.
本研究的目的是比较胚胎期和出生后早期雏鸡和大鼠背根神经节神经元的形态发育。在去除被膜和结缔组织后，通过扫描电子显微镜观察原位神经节和分离神经元中神经元的三维结构。确定了不同发育阶段神经元的百分比。雏鸡的一般形态变化与大鼠相似，但时间不同。在雏鸡和大鼠中，大多数神经元在发育早期（雏鸡胚胎第6天和大鼠第14天）为双极神经元，随后经历假单极化，在孵化或出生时大多成为单极神经元。这种成熟过程在雏鸡胚胎中比在大鼠中开始得更早，在胚胎第14天，雏鸡中有57%的单极神经元，而大鼠中只有7%。然而，在孵化或出生后，即发育第22天，雏鸡中未成熟单极神经元的比例（13%）高于大鼠（3%）。我们得出结论，在设计组织培养中生长的背根神经节神经元实验时，应考虑这些差异。
