McGinnis M Y, Katz S E
Department of Cell Biology and Anatomy, Mount Sinai School of Medicine, New York, NY 10029, USA.
J Neuroendocrinol. 1996 Mar;8(3):193-7. doi: 10.1046/j.1365-2826.1996.04494.x.
The purpose of this experiment was to determine if there are sex differences in cytosolic androgen receptors (AR) in individual brain nuclei. Bilateral 500 mu diameter samples from 300 mu thick frozen brain sections were micropunched from males and females 2-3 weeks following gonadectomy. Tissue samples were taken from 12 brain nuclei: lateral septum, bed nucleus of the stria terminalis, medial preoptic nucleus, anterior hypothalamic area, arcuate nucleus, corticomedial nucleus of the amygdala, lateral preoptic area, parietal cortex, medial nucleus of the amygdala, dorsomedial nucleus of the hypothalamus, ventromedial nucleus of the hypothalamus, and dorsal hippocampus. Cytosolic ARs were found in brain regions involved in the neuromodulation of androgen dependent responses. These data suggest that behavioral differences in male reproductive responses may be the sexually dimorphic distribution of androgen receptor containing neurons.
本实验的目的是确定在个体脑核的胞质雄激素受体（AR）中是否存在性别差异。在去势后2 - 3周，从雄性和雌性大鼠300微米厚的冷冻脑切片上，用微穿孔法获取直径500微米的双侧样本。组织样本取自12个脑核：外侧隔核、终纹床核、内侧视前核、下丘脑前区、弓状核、杏仁核皮质内侧核、外侧视前区、顶叶皮质、杏仁核内侧核、下丘脑背内侧核、下丘脑腹内侧核和背侧海马体。在参与雄激素依赖性反应神经调节的脑区发现了胞质AR。这些数据表明，雄性生殖反应中的行为差异可能是含雄激素受体神经元的性别二态性分布所致。
