Soriano M A, Ferrer I, Rodríguez-Farré E, Planas A M
Department de Farmacologia i Toxicologia, CID-CSIC, Hospital Prínceps d'Espanya, Universitat de Barcelona, Spain.
Neuroreport. 1996 Jan 31;7(2):425-8. doi: 10.1097/00001756-199601310-00012.
Cortical infarction produces secondary neuronal damage in the thalamus. In this study we examined the thalamus of the rat following 2 h occlusion of the middle cerebral artery (MCA), and found degeneration and gliosis in the ipsilateral ventropostero-medial thalamic nucleus in those rats that showed cortical infarction 7 and 14 days after occlusion. This was accompanied by isolated cells with fragmented DNA, as revealed by in situ labelling of nuclear DNA fragmentation, and showing morphological features of apoptosis, i.e. chromatin condensation, extreme nuclear shrinkage and apoptotic bodies. In addition, cells immunoreactive for c-Jun showing morphological signs of apoptosis were observed. These results provide evidence of apoptosis in the ipsilateral thalamus following cortical infarction, and suggest that c-Jun is involved in this process.
皮质梗死会在丘脑产生继发性神经元损伤。在本研究中，我们对大脑中动脉（MCA）闭塞2小时后的大鼠丘脑进行了检查，发现在闭塞后7天和14天出现皮质梗死的大鼠中，其同侧腹后内侧丘脑核发生了变性和胶质增生。如通过核DNA片段原位标记所揭示的，这伴随着具有DNA片段化的单个细胞，且呈现出凋亡的形态学特征，即染色质浓缩、细胞核极度缩小和凋亡小体。此外，观察到对c-Jun呈免疫反应的细胞表现出凋亡的形态学迹象。这些结果提供了皮质梗死后同侧丘脑发生凋亡的证据，并表明c-Jun参与了这一过程。
