Swarzenski B C, O'Malley K L, Todd R D
Department of Psychiatry, Washington University School of Medicine, St. Louis, MO 63110, USA.
Neuroreport. 1996 Jan 31;7(2):573-6. doi: 10.1097/00001756-199601310-00047.
Neurotransmitter receptors are known to have direct roles in the modulation of neuronal morphogenesis. Previous work showed that clonal mesencephalic MN9D cells individually transfected with D2-like dopamine receptors show increased neurite outgrowth following long-term exposure to the D2-like receptor agonist quinpirole. In the current study, brief stimulation of D3 receptor-expressing cells also elicited increased neurite outgrowth, which could be mimicked by the Gi/G(o) protein activator mastoparan. Pretreatment with the Gi/G(o) protein inhibitor pertussis-toxin blocked the quinpirole- and mastoparan-mediated increases in outgrowth. These results suggest that dopamine D3 receptor stimulation has an immediate, G-protein-mediated role in neuronal morphogenesis.
已知神经递质受体在神经元形态发生的调节中具有直接作用。先前的研究表明，单独转染D2样多巴胺受体的中脑克隆MN9D细胞在长期暴露于D2样受体激动剂喹吡罗后，神经突生长增加。在本研究中，短暂刺激表达D3受体的细胞也会引起神经突生长增加，这可以被Gi/G(o)蛋白激活剂马斯托帕罗模拟。用Gi/G(o)蛋白抑制剂百日咳毒素预处理可阻断喹吡罗和马斯托帕罗介导的生长增加。这些结果表明，多巴胺D3受体刺激在神经元形态发生中具有直接的、G蛋白介导的作用。
