Paulsen I T, Skurray R A, Tam R, Saier M H, Turner R J, Weiner J H, Goldberg E B, Grinius L L
School of Biological Sciences, University of Sydney, Australia.
Mol Microbiol. 1996 Mar;19(6):1167-75. doi: 10.1111/j.1365-2958.1996.tb02462.x.
The sequenced members of a novel family of small, hydrophobic, bacterial multidrug-resistance efflux proteins, which we have designated the small multidrug resistance (SMR) protein family, are identified and analysed. Two distinct clusters of proteins were identified within this family: (i) small multidrug efflux systems; and (ii) Sug proteins, potentially involved in the suppression of groEL mutations. Hydropathy and residue distribution analyses of this family suggest a structural model in which the polypeptide chain spans the membrane four times as mildly amphipathic alpha-helices. The roles of specific residues, a possible mechanistic model of drug efflux, and the primary physiological role(s) of the SMR proteins are discussed.
我们已将其命名为小多药耐药（SMR）蛋白家族的一类新型小的、疏水的细菌多药耐药外排蛋白的测序成员得以鉴定和分析。在该家族中鉴定出了两个不同的蛋白簇：（i）小多药外排系统；以及（ii）可能参与抑制groEL突变的Sug蛋白。对该家族的亲水性和残基分布分析提示了一种结构模型，其中多肽链以轻度两亲性α螺旋四次跨越细胞膜。文中讨论了特定残基的作用、药物外排可能的机制模型以及SMR蛋白的主要生理作用。
