通过辐射失活法估算海盘车（Asterias amurensis）中顶体反应诱导物质（ARIS）的最小功能大小。

Estimation by radiation inactivation of the minimum functional size of acrosome-reaction-inducing substance (ARIS) in the starfish, Asterias amurensis.

作者信息

Ushiyama A, Chiba K, Shima A, Hoshi M

机构信息

Tokyo Institute of Technology, Yokohama, Japan.

出版信息

Zygote. 1995 Nov;3(4):351-5. doi: 10.1017/s0967199400002781.

DOI:10.1017/s0967199400002781

PMID:8730900

Abstract

In the starfish Asterias amurensis, the jelly coat of the eggs contains a glycoprotein essential for the induction of the acrosome reaction in homologous spermatozoa that is termed the acrosome-reaction-inducing substance (ARIS). ARIS is a highly sulphated and fucose-rich glycoprotein of extremely high molecular mass (> 10(4) kDa). ARIS was irradiated with high-energy electrons in order to estimate the minimum size required for its biological activity. The minimum functional unit or target size of ARIS was estimated to be c. 14 kDa by target size analysis. ARIS was significantly disintegrated by the irradiation, yet the total sugar content was not apparently reduced. The binding of 125I-labelled ARIS to spermatozoa competed with that of irradiated ARIS, although the affinity of ARIS was much reduced after irradiation.

摘要

在多棘海盘车（Asterias amurensis）中，卵的卵黄膜含有一种糖蛋白，它对诱导同源精子发生顶体反应至关重要，这种糖蛋白被称为顶体反应诱导物质（ARIS）。ARIS是一种高度硫酸化且富含岩藻糖的糖蛋白，分子量极高（>10⁴ kDa）。为了估计其生物活性所需的最小尺寸，用高能电子对ARIS进行辐照。通过靶尺寸分析，ARIS的最小功能单位或靶尺寸估计约为14 kDa。辐照使ARIS显著分解，但总糖含量并未明显降低。¹²⁵I标记的ARIS与精子的结合能被辐照后的ARIS竞争，尽管辐照后ARIS的亲和力大幅降低。