Romay C, Pascual C, Lissi E A
Centro Nacional de Investigaciones Científicas, La Habana, Cuba.
Braz J Med Biol Res. 1996 Feb;29(2):175-83.
The 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) (ABTS) radical cation can be generated by incubation of ABTS and 2,2'-azo-bis(2- amidinopropane) at 45 degrees C. The ABTS radical cation is stable for several minutes at room temperature and reacts quantitatively and instantaneously with several antioxidants, such as Trolox, ascorbic acid, uric acid, cysteine, glutathione and bilirubin. In contrast, the ABTS radical cation reacts slowly with albumin. When serum is added to a solution of the ABTS radical cation, the bleaching of the radical follows biphasic kinetics, with a fast decay followed by a slow decay that takes place within several minutes. The fast decay is primarily due to uric acid, while the slow decay is related to the protein content of the sample. We propose that this procedure can provide an independent and simultaneous evaluation of the low molecular weight and protein antioxidants present in biological samples such as serum.
2,2'-联氮-双(3-乙基苯并噻唑啉-6-磺酸)（ABTS）自由基阳离子可通过在45℃下孵育ABTS和2,2'-偶氮-双(2-脒基丙烷)生成。ABTS自由基阳离子在室温下几分钟内稳定，并与几种抗氧化剂（如Trolox、抗坏血酸、尿酸、半胱氨酸、谷胱甘肽和胆红素）发生定量且即时的反应。相比之下，ABTS自由基阳离子与白蛋白反应缓慢。当向ABTS自由基阳离子溶液中加入血清时，自由基的褪色遵循双相动力学，先是快速衰减，随后在几分钟内缓慢衰减。快速衰减主要归因于尿酸，而缓慢衰减与样品中的蛋白质含量有关。我们认为该方法可以对血清等生物样品中存在的低分子量和蛋白质抗氧化剂进行独立且同时的评估。
