Bartosz G, Janaszewska A, Ertel D, Bartosz M
Department of Molecular Biophysics, University of Lódź, Poland.
Biochem Mol Biol Int. 1998 Oct;46(3):519-28. doi: 10.1080/15216549800204042.
A simple spectrophotometric method of determination of peroxyl radical-trapping capacity (PRTC) of body fluids and food products is proposed. In this method, decomposition of 2,2'-azobis(2-amidopropane) hydrochloride (ABAP) is the source of peroxyl and alkoxyl radicals which oxidize 2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) to a green cation radical. Antioxidant present in a sample inhibit the reaction; the induction time of the reaction is proposed as a parameter enabling determination of antioxidant content. Standard assay conditions are: 20 mM ABAP and 150 microM ABTS in 0.1 M phosphate buffer, pH 7.0, at 37 degrees C; absorbance is monitored at 414 nm. A 10-min assay allows for determination of the induction time of appropriately diluted sample. As examples of application of this method, PRTC values of several types of beverages are reported.
本文提出了一种测定体液和食品过氧自由基捕获能力（PRTC）的简单分光光度法。在该方法中，盐酸2,2'-偶氮二（2-氨丙烷）（ABAP）的分解是过氧自由基和烷氧自由基的来源，这些自由基将2,2'-偶氮二（3-乙基苯并噻唑啉-6-磺酸）（ABTS）氧化为绿色阳离子自由基。样品中存在的抗氧化剂会抑制该反应；建议将反应的诱导时间作为测定抗氧化剂含量的参数。标准测定条件为：在0.1 M磷酸盐缓冲液（pH 7.0）中，37℃下，20 mM ABAP和150 μM ABTS；在414 nm处监测吸光度。10分钟的测定可确定适当稀释样品的诱导时间。作为该方法应用的示例，报告了几种类型饮料的PRTC值。
