Sydserff S G, Green A R, Cross A J
Astra Neuroscience Research Unit, London.
Neurodegeneration. 1996 Mar;5(1):81-5. doi: 10.1006/neur.1996.0011.
The effect of chlormethiazole on hemispheric swelling and cortical tissue water content has been investigated in a model of permanent middle cerebral artery (MCA) occlusion. Chlormethiazole (1 mmol/kg i.p.) or saline was administered 60 min after the induction of ischaemia and the animals sacrificed after 24 hours. The cross sectional area of the left hemisphere was increased by 21.8 + 1.9% in saline treated rats, but only by 8.4 + 2.4% in chlormethiazole treated rats. However, the reduction in the absolute area of neurodegenerative damage (mm2) following chlormethiazole administration was considerably greater than the reduction in hemispheric swelling. Cortical tissue water content of ischaemic brain increased from 76.4% to 84.2% and this was attenuated to 78.8% by chlormethiazole administration. These data demonstrate that, providing damage is measured by fitting tissue slices onto prematched stereotactic maps, the decrease in oedema which accompanies a decrease in neurodegeneration does not result in erroneous estimates of neuroprotection.
在大脑中动脉（MCA）永久性闭塞模型中，研究了氯美噻唑对半球肿胀和皮质组织含水量的影响。缺血诱导60分钟后，腹腔注射氯美噻唑（1 mmol/kg）或生理盐水，24小时后处死动物。生理盐水处理的大鼠左半球横截面积增加了21.8 + 1.9%，而氯美噻唑处理的大鼠仅增加了8.4 + 2.4%。然而，给予氯美噻唑后神经退行性损伤的绝对面积（mm2）减少量明显大于半球肿胀的减少量。缺血性脑皮质组织含水量从76.4%增加到84.2%，而给予氯美噻唑后该含水量减至78.8%。这些数据表明，如果通过将组织切片拟合到预先匹配的立体定向图谱上来测量损伤，那么伴随神经退行性变减少的水肿减少不会导致对神经保护的错误估计。
