Technicon Immuno 1前列腺特异性抗原检测法可在等摩尔基础上检测游离的和与α1抗糜蛋白酶结合的前列腺特异性抗原。
Technicon Immuno 1 PSA assay measures both free and alpha-1-antichymotrypsin-complexed prostate-specific antigen on an equimolar basis.
作者信息
Zhou Z, Ng P C, Very D L, Allard W J, Yeung K K
机构信息
Immunodiagnostics Business Unit, Bayer Corporation, Tarrytown, New York 10591-5097, USA.
出版信息
J Clin Lab Anal. 1996;10(3):155-9. doi: 10.1002/(SICI)1098-2825(1996)10:3<155::AID-JCLA8>3.0.CO;2-A.
In this study, we investigated the immunoreactivity of the Technicon Immuno 1 PSA assay, a monoclonal-polyclonal sandwich immunoassay, with free and ACT-complexed PSA. Assay calibrators prepared with free PSA (standard Immuno 1 calibrators) and calibrators consisting of 90% ACT-complexed and 10% free PSA (90:10 calibrators) yielded virtually identical PSA recoveries at all concentrations tested. Concentrations of total PSA at approximately 4 and 10 ng/mL, prepared in varying ratios of free PSA to PSA-ACT complex, recovered from 92-104% at 4 ng/mL and from 98-102% at 10 ng/mL. Additionally, an excellent correlation of serum total PSA values from a panel of 40 prostatic cancer patient samples was obtained using calibration curves generated with the standard Immuno 1 calibrators or the 90:10 calibrators. These results demonstrate that the Technicon Immuno 1 PSA assay measures free and ACT-complexed PSA on an equal molar basis.
在本研究中，我们调查了Technicon Immuno 1 PSA检测法（一种单克隆 - 多克隆夹心免疫测定法）对游离PSA和与α1 - 抗糜蛋白酶（ACT）结合的PSA的免疫反应性。用游离PSA制备的检测校准品（标准Immuno 1校准品）以及由90%与ACT结合的PSA和10%游离PSA组成的校准品（90:10校准品）在所有测试浓度下产生的PSA回收率几乎相同。以游离PSA与PSA - ACT复合物的不同比例制备的总PSA浓度约为4和10 ng/mL时，4 ng/mL的回收率为92 - 104%，10 ng/mL的回收率为98 - 102%。此外，使用标准Immuno 1校准品或90:10校准品生成的校准曲线，对40例前列腺癌患者样本的血清总PSA值进行检测，结果具有良好的相关性。这些结果表明，Technicon Immuno 1 PSA检测法在等摩尔基础上测定游离PSA和与ACT结合的PSA。
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