Ruan H Z, Li X C, Cai W Q
Department of Physiology, Third Military Medical College, Chongqing, China.
Zhongguo Yao Li Xue Bao. 1995 Nov;16(6):512-6.
To study the effect of serotonin (5-HT) on pain modulation of substance P (SP) in spinal cord of rats.
Using immunohisto-chemistry and measurement of pain threshold.
The c-fos expression evoked by intrathecal injection (it) SP 10 micrograms and sc 5% formaldehyde (For) 150 microL in the hindpaw was densely distributed in the laminae I, II, V, and VI of spinal dorsal horn. The pain threshold in the SP group was decreased while the pain intensity rating measured by behavioral method in the For group was increased. The c-fos expression induced by it 5-HT 20 micrograms was mostly distributed in the spinal dorsal horn in laminae III-IV and the pain threshold was increased. SP and For induced c-fos expressions in the spinal cord and the pain responses were reduced by 5-HT and increased by 5-HT depletor fenclonine 300 mg.kg-1.
SP mainly played an algogenesia in the spinal cord. 5-HT inhibited the c-fos expression in the spinal cord evoked by SP and participated in pain modulation of SP.
研究5-羟色胺（5-HT）对大鼠脊髓中P物质（SP）痛觉调制的影响。
采用免疫组织化学和痛阈测量法。
鞘内注射10微克SP和后爪皮下注射150微升5%甲醛（For）所诱发的c-fos表达密集分布于脊髓背角的I、II、V和VI层。SP组的痛阈降低，而For组通过行为学方法测得的疼痛强度评分增加。鞘内注射20微克5-HT所诱导的c-fos表达主要分布在脊髓背角的III-IV层，且痛阈升高。SP和For诱导脊髓中的c-fos表达，5-HT可减轻疼痛反应，而5-HT耗竭剂300毫克·千克-1的芬克洛宁则增强疼痛反应。
SP在脊髓中主要起致痛作用。5-HT抑制SP诱发的脊髓中c-fos表达，并参与SP的痛觉调制。
