Wang J, Li G, Liu Z
Department of Pathology, West China University of Medical Sciences, Chengdu.
Zhonghua Bing Li Xue Za Zhi. 1995 Dec;24(6):337-40.
Polymerase chain reaction (PCR) and immunohistochemical staining methods were used to detect the bcl-2/IgH fusion gene [t(14:18)] and the overexpression of bcl-2 protein in 28 cases of follicular lymphomas and 18 cases of reactive follicular hyperplasia. The results showed that the bcl-2/IgH fusion gene was present in 57% of follicular lymphomas and that this translocation was also present in 33% of the reactive follicular hyperplasia cases. The over expression of bcl-2 protein was found in 78% of follicular lymphomas, but none in follicular hyperplasia, therefore bcl-2 protein staining may be a useful immunohistochemical method to differentiate follicular lymphoma and follicular hyperplasia.
采用聚合酶链反应（PCR）和免疫组化染色方法检测28例滤泡性淋巴瘤及18例反应性滤泡增生中bcl-2/IgH融合基因[t(14;18)]及bcl-2蛋白的过表达情况。结果显示，57%的滤泡性淋巴瘤存在bcl-2/IgH融合基因，33%的反应性滤泡增生病例也存在这种易位。78%的滤泡性淋巴瘤中发现bcl-2蛋白过表达，而滤泡增生中未发现，因此bcl-2蛋白染色可能是鉴别滤泡性淋巴瘤和滤泡增生的一种有用的免疫组化方法。
