Li J, Yang G, Li G
Department of Pathology, West China University of Medical Sciences, Chengdu.
Zhonghua Zhong Liu Za Zhi. 1996 Jan;18(1):13-5.
Silver staining PCR-SSCP method was used to detect point mutation of p53 gene in paraffin-embedded malignant fibrous histiocytoma (MFH) tissues. The abnormal shifting of the single-stranded DNA (ssDNA) was identified in 9 out of 16 cases. The positive figure of SSCP was 1,4,4, 3 in exon 5, 6, 7, 8, respectively. The mutant p53 protein was detected by microwave oven treatment and ABC immunohistochemistry. Positive nuclear staining was observed in 10 cases. The positive coincidence rate was 90.0% between SSCP and p53 protein expression. The mutation of p53 gene was not correlated with the subtypes of MFH. Our results indicate that detection of point mutations with silver staining PCR-SSCP is convenient, rapid and reliable in the screening of point mutation of genes.
采用银染PCR-SSCP法检测石蜡包埋的恶性纤维组织细胞瘤（MFH）组织中p53基因的点突变。16例中有9例鉴定出单链DNA（ssDNA）异常迁移。SSCP在外显子5、6、7、8中的阳性数分别为1、4、4、3。采用微波炉处理和ABC免疫组织化学法检测突变型p53蛋白。10例观察到细胞核阳性染色。SSCP与p53蛋白表达的阳性符合率为90.0%。p53基因的突变与MFH的亚型无关。我们的结果表明，银染PCR-SSCP检测点突变在基因点突变筛查中方便、快速且可靠。
