Dong Y, Liu S
Cancer Institute, Chinese Academy of Medical Sciences, Beijing.
Zhonghua Zhong Liu Za Zhi. 1996 Jan;18(1):58-60.
The levels of p53 protein were detected by immunocytochemistry in 197 endoscopic biopsy specimens of esophagus. Nuclear p53 protein was present in 2.9% of normal mucosa, 6.7% of inflammatory mucosa, 38.8% of mild dysplasia, 52.0% of moderate or severe dysplasia, 61.1% of carcinomas in situ, 62.5% of invasive carcinomas. Moreover, we examined 14 cases of esophageal carcinoma with extensive dysplasia near the carcinoma and found high levels of p53 protein in both dysplasias and carcinomas in 8 cases. In one case, carcinoma presented high level of p53 protein while the adjacent dysplasia did not. In another case, the result was just the opposite. Negativity for p53 immunostaining was found in 4 cases. These data indicated that p53 protein accumulation occurred before tumor invasion in the multistage esophageal carcinogenesis. The timing and the frequency of p53 protein accumulation made p53 gene an attractive marker for the early diagnosis an the evaluation of chemopreventive agents.
采用免疫细胞化学方法检测了197例食管内镜活检标本中p53蛋白的水平。核p53蛋白在2.9%的正常黏膜、6.7%的炎性黏膜、38.8%的轻度不典型增生、52.0%的中度或重度不典型增生、61.1%的原位癌、62.5%的浸润癌中存在。此外，我们检查了14例癌旁有广泛不典型增生的食管癌，发现8例不典型增生和癌中p53蛋白水平均较高。1例中，癌呈现p53蛋白高水平而相邻的不典型增生未出现。另1例结果相反。4例p53免疫染色为阴性。这些数据表明在食管多阶段癌变过程中p53蛋白积累发生在肿瘤浸润之前。p53蛋白积累的时间和频率使p53基因成为早期诊断和化学预防药物评估的一个有吸引力的标志物。
