Haedersdal M, Wulf H C
Department of Dermatology, National University Hospital, Rigshospitalet, Copenhagen, Denmark.
Lasers Surg Med. 1996;18(4):391-6. doi: 10.1002/(SICI)1096-9101(1996)18:4<391::AID-LSM8>3.0.CO;2-N.
Port wine stains are commonly located on UV-exposed skin areas. We therefore examined the long-term interaction between UV radiation and copper vapor laser light (578 nm, yellow light) and whether the thermal influence from laser light had a carcinogenic potential itself.
STUDY DESIGN/MATERIALS AND METHODS: The study was conducted in lightly pigmented hairless hr/hr C3H/Tif mice and included 8 groups of 17-20 mice. Intensities of 0.5, 1.0, and 1.4 W were used, corresponding to calibrated Hexascan fluences of 15.9, 31.8, and 44.6 J/cm2. Beam diameter was 1 mm and pulse duration 250 msec. UV irradiation of the mice was performed 4 days weekly and started the day after laser treatment. The UV simulated solar ultraviolet radiation can from one Phillips TL 12 and five Bellarium-S SA-1-12 tubes. The daily dose was 1.3 J/cm2, equivalent to 2.1 B-MED.
No tumors appeared in groups receiving laser light only. The time to first (P < 0.01), second (P < 0.01), and third (P < 0.02) tumor was significantly delayed in the group treated with 1.4 W before UV irradiation (P < 0.01) compared with those receiving UV radiation only. No significant differences could be demonstrated for the groups treated with 0.5 and 1.0 W.
One laser treatment with the copper vapor laser did not accelerate UV-induced photocarcinogenesis, and the laser exposure did not have a malignant potential itself.
葡萄酒色斑通常位于暴露于紫外线的皮肤区域。因此，我们研究了紫外线辐射与铜蒸气激光（578纳米，黄光）的长期相互作用，以及激光产生的热影响本身是否具有致癌潜力。
研究设计/材料与方法：该研究在色素沉着较浅的无毛hr/hr C3H/Tif小鼠中进行，包括8组，每组17 - 20只小鼠。使用的强度为0.5、1.0和1.4瓦，分别对应校准后的Hexascan能量密度15.9、31.8和44.6焦/平方厘米。光束直径为1毫米，脉冲持续时间为250毫秒。小鼠每周接受4天紫外线照射，在激光治疗后的第二天开始。紫外线模拟太阳紫外线辐射由一根飞利浦TL 12灯管和五根Bellarium - S SA - 1 - 12灯管提供。每日剂量为1.3焦/平方厘米，相当于2.1个生物等效剂量。
仅接受激光照射的组未出现肿瘤。与仅接受紫外线辐射的组相比，在紫外线照射前接受1.4瓦激光治疗的组中，首次出现肿瘤的时间（P < 0.01）、第二次出现肿瘤的时间（P < 0.01）和第三次出现肿瘤的时间（P < 0.02）均显著延迟（P < 0.01）。接受0.5瓦和1.0瓦激光治疗的组之间未显示出显著差异。
铜蒸气激光单次治疗不会加速紫外线诱导的光致癌作用，且激光照射本身不具有恶性潜能。
