Podda M, Weber C, Traber M G, Packer L
Department of Molecular and Cell Biology, University of California, Berkeley 94720-3200, USA.
J Lipid Res. 1996 Apr;37(4):893-901.
A tissue-specific distribution of the various vitamin E forms, tocotrienols and tocopherols, has been found, suggesting that these forms have unique roles in cellular functions. A sensitive procedure is described for the simultaneous determination of individual tocopherols, tocotrienols, ubiquinols, and ubiquinones using gradient high pressure liquid chromatography (HPLC) and electrochemical detection for vitamin E homologues and ubiquinols, and in-line UV detection for ubiquinones. Using this method, the lipophilic antioxidant complement of a variety of hairless mouse tissues was analyzed. Of the vitamin E forms, brain contained virtually only alpha-tocopherol (5.4 +/- 0.1 nmol/g; 99.8%) and no detectable tocotrienols were found. By contrast, skin contained nearly 15% tocotrienols and 1% gamma-tocopherol. In other tissues, the alpha-tocopherol content was higher (20 nmol/g), while each of the other forms represented about 1% of the total (gamma-tocopherol 0.2 to 0.4 nmol/g, alpha-tocotrienol 0.1, gamma-tocotrienol 0.2). Ubiquinol-9 concentrations were highest in kidney (81 nmol/g) and in liver (42 nmol/g), while the highest ubiquinone-9 concentrations were found in kidney (301 +/- 123 nmol/g) and heart (244 +/- 22 nmol/g). Liver contained nearly identical concentrations of each of the redox couple (ubiquinol-9 (41 +/- 16 nmol/g) and ubiquinone-9 (46 +/- 18 nmol/g). The unique distribution of these various antioxidants in the tissues measured suggests their distribution may be dependent upon selective mechanisms for maintaining antioxidant defenses in each tissue.
已发现各种维生素E形式（生育三烯酚和生育酚）存在组织特异性分布，这表明这些形式在细胞功能中具有独特作用。本文描述了一种灵敏的方法，使用梯度高压液相色谱（HPLC）以及针对维生素E同系物和泛醇的电化学检测，和针对泛醌的在线紫外检测，同时测定各生育酚、生育三烯酚、泛醇和泛醌。使用该方法，分析了多种无毛小鼠组织的亲脂性抗氧化剂组成。在维生素E形式中，大脑几乎只含有α-生育酚（5.4±0.1 nmol/g；99.8%），未检测到生育三烯酚。相比之下，皮肤含有近15%的生育三烯酚和1%的γ-生育酚。在其他组织中，α-生育酚含量较高（20 nmol/g），而其他每种形式约占总量的1%（γ-生育酚0.2至0.4 nmol/g，α-生育三烯酚0.1，γ-生育三烯酚0.2）。泛醇-9浓度在肾脏（81 nmol/g）和肝脏（42 nmol/g）中最高，而泛醌-9浓度在肾脏（301±123 nmol/g）和心脏（244±22 nmol/g）中最高。肝脏中氧化还原对的每种成分浓度几乎相同（泛醇-9（41±16 nmol/g）和泛醌-9（46±18 nmol/g）。所测组织中这些各种抗氧化剂的独特分布表明，它们的分布可能取决于维持各组织抗氧化防御的选择性机制。
