Parker K L, Ikeda Y, Luo X
Department of Medicine, Duke University Medical Center, Durham, North Carolina, USA.
Steroids. 1996 Apr;61(4):161-5. doi: 10.1016/0039-128x(96)00006-2.
The cytochrome P450 steroid hydroxylases are expressed in a tissue-specific and developmentally regulated manner, and the orphan nuclear receptor steroidogenic factor 1 (SF-1) participates in both aspects of regulated expression. SF-1 is expressed in mouse embryos from the inception of adrenal and gonadal development, suggesting that SF-1 plays important roles in their differentiation. SF-1 is also expressed in the embryonic pituitary gland and ventral diencephalon, suggesting additional roles within the hypothalamic-pituitary-steroidogenic organ axis. To examine the roles of SF-1 in vivo, we used targeted gene disruption to "knock out" the mouse gene encoding SF-1. Analyses of these knockout mice established roles of SF-1 at levels of endocrine development that include adrenal and gonadal differentiation, pituitary gonadotrope function, and formation of the ventromedial hypothalamic nucleus. These results indicate that SF-1 plays multiple roles in endocrine development that are essential for reproduction.
细胞色素P450类固醇羟化酶以组织特异性和发育调控的方式表达，孤儿核受体类固醇生成因子1（SF-1）参与调控表达的两个方面。从肾上腺和性腺发育开始，SF-1就在小鼠胚胎中表达，这表明SF-1在它们的分化中起重要作用。SF-1也在胚胎垂体和腹侧间脑中表达，提示其在下丘脑-垂体-类固醇生成器官轴中还有其他作用。为了研究SF-1在体内的作用，我们利用靶向基因敲除技术“敲除”了编码SF-1的小鼠基因。对这些基因敲除小鼠的分析确定了SF-1在内分泌发育水平上的作用，包括肾上腺和性腺分化、垂体促性腺激素细胞功能以及腹内侧下丘脑核的形成。这些结果表明，SF-1在对生殖至关重要的内分泌发育中发挥多种作用。
