Johnson A G, Simons L A, Friedlander Y, Simons J, Davis D R, MaCallum J
University Department of Medicine, Princess Alexandra Hospital, Brisbane, Australia.
J Hum Hypertens. 1996 Mar;10(3):167-9.
To determine whether insertion/deletion (I/D) polymorphism (intron 16) of the angiotensin converting-enzyme (ACE) gene is associated with isolated systolic hypertension (ISH: systolic blood pressure (BP) > or = 160, diastolic BP < 90 mm Hg) or systolic-diastolic hypertension (S-D hypertension: diastolic BP > or = 90 +/- systolic BP > or = 160 mm Hg) compared with normotensive controls (systolic BP < 160, diastolic BP < 90 mm Hg), we conducted a case-control study of 733 non-institutionalised, elderly (> or = 60 years) residents of Dubbo, NSW. Individuals were classified as: ISH (n = 167), S-D hypertension (n = 207) and normotensive control (n = 359) with age and sex matching. II, DD and ID genotypes were determined by a nested PCR strategy using DNA extracted from serum. The frequencies of D and I alleles in the control population (0.70 and 0.30 respectively) were not significantly different in the ISH group or the S-D hypertension group (chi 2: 1.7, P = 0.42). After adjustment for several potential confounders, neither genotype nor allele predicted ISH (II vs DD: odds ratio (OR): 1.06, 95% confidence interval (CI): 0.55-2.03; I vs D: 1.09, 0.82-1.46) or S-D hypertension (II vs DD: 1.19, 0.67-2.10; I vs D: 1.16, 0.89-1.52) in this elderly cohort. The I/D polymorphism of the ACE gene is not a marker for either form of hypertension in this large elderly sample.
为了确定血管紧张素转换酶（ACE）基因的插入/缺失（I/D）多态性（第16内含子）与单纯收缩期高血压（ISH：收缩压（BP）≥160，舒张压BP＜90 mmHg）或收缩压-舒张压高血压（S-D高血压：舒张压BP≥90±收缩压BP≥160 mmHg）是否与血压正常的对照组（收缩压BP＜160，舒张压BP＜90 mmHg）相关，我们对新南威尔士州达博市733名非住院的老年（≥60岁）居民进行了一项病例对照研究。个体被分类为：ISH（n = 167）、S-D高血压（n = 207）和血压正常对照组（n = 359），并进行年龄和性别匹配。使用从血清中提取的DNA，通过巢式PCR策略确定II、DD和ID基因型。对照组人群中D和I等位基因的频率（分别为0.70和0.30）在ISH组或S-D高血压组中无显著差异（χ2：1.7，P = 0.42）。在对几个潜在混杂因素进行调整后，在这个老年队列中，基因型和等位基因均不能预测ISH（II与DD：优势比（OR）：1.06，95%置信区间（CI）：0.55 - 2.03；I与D：1.09，0.82 - 1.46）或S-D高血压（II与DD：1.19，0.67 - 2.10；I与D：1.16，0.89 - 1.52）。在这个大型老年样本中，ACE基因的I/D多态性不是任何一种高血压形式的标志物。
