Gerritsen E J, van Tol M J, Ballieux P, Voesten A, Weiland H T, van Venrooij W J, Daha M R, Geertzen H G, Rijkers G T, Gmelig-Meyling F H, Claas F J, Radl J, Vossen J M
Department of Paediatrics, University Hospital, Leiden, The Netherlands.
Bone Marrow Transplant. 1996 May;17(5):825-33.
After allogeneic BMT, transient homogeneous Ig components (H-Ig) can be detected in the sera of most graft recipients. So far, data on the antigen-specificity and therefore the function of these H-Ig are not available. Such information may be important for our understanding of the underlying mechanisms that are responsible for these excessive clonal B cell expansions, and it may help to delineate the functional antibody repertoire after BMT. In the present study, sera of 98 paediatric BM graft recipients were investigated for the potential presence of H-Ig of IgG isotype (H-IgG) with specificity towards a panel of antigens, including vaccine and herpes virus antigens, auto-antigens and allo-antigens. The vast majority of H-IgG in sera of BM graft recipients were unreactive when tested for this panel of antigens. However, in four cases, antigen-specificity of H-IgG to tetanus toxoid could be demonstrated after vaccination with that antigen. An explanation for the negative findings may be either that a restricted antibody production had been elicited by other non-tested antigens, eg substances of colonizing and translocating bacteria or of food antigens, or that the H-IgG components may have anti-idiotype or anti-'self' specificity.
在同种异体骨髓移植后，大多数移植受者的血清中可检测到短暂的同源免疫球蛋白成分（H-Ig）。到目前为止，关于这些H-Ig的抗原特异性及其功能的数据尚不可得。此类信息对于我们理解导致这些过度克隆性B细胞扩增的潜在机制可能很重要，并且可能有助于描绘骨髓移植后的功能性抗体库。在本研究中，对98名儿科骨髓移植受者的血清进行了检测，以寻找是否存在针对一组抗原（包括疫苗和疱疹病毒抗原、自身抗原和同种异体抗原）具有特异性的IgG同种型H-Ig（H-IgG）。当对这组抗原进行检测时，骨髓移植受者血清中的绝大多数H-IgG没有反应。然而，在4例病例中，接种破伤风类毒素疫苗后，可证明H-IgG对破伤风类毒素具有抗原特异性。阴性结果的一个解释可能是，其他未检测的抗原（例如定居和移位细菌的物质或食物抗原）引发了有限的抗体产生，或者H-IgG成分可能具有抗独特型或抗“自身”特异性。
