Imagawa A, Itoh N, Hanafusa T, Katsura H, Yamaguchi A, Takeda A, Iwahashi H, Nakajima H, Miyagawa J, Namba M, Kuwajima M, Matsuzawa Y
Second Department of Internal Medicine, Osaka University Medical School, Suita, Japan.
Res Commun Mol Pathol Pharmacol. 1996 Apr;92(1):43-52.
To elucidate the clinical significance of antibodies to glutamic acid decarboxylase (GAD Ab) compared to islet cell antibodies (ICA) in recent-onset and long-standing insulin-dependent diabetes mellitus (IDDM). We examined GAD Ab and ICA in 29 recent-onset and 85 long-standing patients with IDDM. GAD Ab was detected by a radioimmunoassay kit using purified pig brain GAD as an antigen. The prevalence of GAD Ab in the recent-onset diabetic patients was 55.2%, slightly lower than that of ICA (65.5%). In contrast, the prevalence of GAD Ab in long-standing diabetic patients was 42.4%, which was significantly higher than that of ICA (23.5%) (p < 0.01). GAD Ab were consistently detected in approximately 40% of patients with long-standing disease, while ICA decreased according to duration of disease. The GAD Ab titer in ICA-positive patients (mean +/- SD, 1588.2 +/- 6755.1; range, 6-38574) was significantly higher than that in ICA-negative patients (mean +/- SD, 13.4 +/- 17.9; and range, 5-72 units) (p < 0.001). These findings suggest that GAD Ab are more useful than ICA to know participation of immune disorders in long-standing patients with IDDM.
为阐明谷氨酸脱羧酶抗体（GAD Ab）相较于胰岛细胞抗体（ICA）在近期发病和病程较长的胰岛素依赖型糖尿病（IDDM）中的临床意义。我们检测了29例近期发病和85例病程较长的IDDM患者的GAD Ab和ICA。使用纯化的猪脑GAD作为抗原，通过放射免疫分析试剂盒检测GAD Ab。近期发病的糖尿病患者中GAD Ab的患病率为55.2%，略低于ICA（65.5%）。相比之下，病程较长的糖尿病患者中GAD Ab的患病率为42.4%，显著高于ICA（23.5%）（p<0.01）。在约40%病程较长的患者中持续检测到GAD Ab，而ICA随病程延长而降低。ICA阳性患者的GAD Ab滴度（均值±标准差，1588.2±6755.1；范围，6 - 38574）显著高于ICA阴性患者（均值±标准差，13.4±17.9；范围，5 - 72单位）（p<0.001）。这些发现表明，在了解病程较长的IDDM患者免疫紊乱的参与情况方面，GAD Ab比ICA更有用。
