Hughes J D, Chen C, Mattar S G, Someren A, Noe B, Suwyn C R, Lumsden A B
Department of Surgery, Emory University School of Medicine, Atlanta, Ga, USA.
Ann Vasc Surg. 1996 Mar;10(2):123-30. doi: 10.1007/BF02000755.
Hypothermia and preservative perfusates have been used to decrease ischemic renal injury. This study was performed to identify the preservative function of perfusates independent of the effects of hypothermia. Rats underwent 45 minutes of renal ischemia. Rectal and renal parenchyma temperatures were monitored and maintained within 1 degree C of normal. Perfusates were University of Wisconsin solution (UW), Euro-Collins solution, normal saline solution, and Ringer's lactate solution. A nonperfused ischemic control and a nonischemic control group were also evaluated. Parameters evaluated included serum creatinine and blood urea nitrogen levels, renal ischemic injury grade, renal weight, and gross appearance of the injured kidney. Rats treated with UW solution were found to have a significantly lower creatinine, blood urea nitrogen, and injury grade than the other three perfused groups. The external gross appearance of the UW-treated kidneys was normal, whereas that of the other groups demonstrated moderate to severe injury. Although the mean right/left renal weight difference of the UW-treated group was lower than that of the other three groups, this was not statistically significant. Under normothermic conditions in rats, UW solution affords significant renal protection from ischemia. Euro-Collins, normal saline, and Ringer's lactate solutions display no significant protective effect.
低温及保存灌注液已被用于减轻缺血性肾损伤。本研究旨在确定灌注液的保护作用，而不考虑低温的影响。对大鼠进行45分钟的肾脏缺血处理。监测直肠及肾实质温度，并使其维持在比正常温度高1摄氏度以内。灌注液为威斯康星大学溶液（UW）、欧洲柯林斯溶液、生理盐水溶液及乳酸林格氏液。还评估了一个未灌注的缺血对照组和一个非缺血对照组。评估的参数包括血清肌酐和血尿素氮水平、肾脏缺血损伤分级、肾脏重量以及受损肾脏的大体外观。发现用UW溶液处理的大鼠的肌酐、血尿素氮及损伤分级显著低于其他三个灌注组。经UW处理的肾脏的外部大体外观正常，而其他组则表现为中度至重度损伤。虽然经UW处理组的左右肾平均重量差异低于其他三组，但这并无统计学意义。在大鼠的常温条件下，UW溶液能为肾脏提供显著的缺血保护作用。欧洲柯林斯溶液、生理盐水及乳酸林格氏液未显示出显著的保护作用。
