Baca-Estrada M E, Snider M, Tikoo S K, Harland R, Babiuk L A, van Drunen Littel-van den Hurk S
Veterinary Infectious Disease Organization, University of Saskatchewan, Saskatoon, Canada.
Viral Immunol. 1996;9(1):11-22. doi: 10.1089/vim.1996.9.11.
For the development of veterinary subunit vaccines, modifications to the antigen may be needed to make the production of these vaccines cost effective. To investigate the effect of antigen modifications on immune response, we used glycoprotein D, one of the major glycoproteins of bovine herpesvirus-1 (BHV-1), as a model antigen. We developed a mouse model to assess the immune response elicited by immunization with either a recombinant truncated (tgD) or the authentic full-length (gD) form of BHV-1 gD in VSA3, a novel water-in-oil adjuvant. Both forms of BHV-1 gD antigen induced good levels of cell-mediated immunity, as evaluated by antigen-specific proliferative response and cytokine (IFN-gamma and IL-4) production. Following primary immunization, the humoral immune response induced by gD was superior to that elicited by vaccination with tgD. However, after a secondary immunization, a strong and similar antibody response to BHV-1 gD was induced by both forms of the antigen. The difference in immunogenicity between gD and tgD after primary immunization was not due to the loss of immunogenic epitopes in the truncated antigen or the ability to associate with the adjuvant VSA3. Our results indicate that both gD and tgD are capable of efficiently inducing a cell-mediated immune response, and although recombinant tgD is less efficient in inducing a primary humoral immune response when compared to the full-length gD, tgD effectively primed for a secondary antibody response.
对于兽用亚单位疫苗的研发，可能需要对抗原进行修饰，以使这些疫苗的生产成本效益更高。为了研究抗原修饰对免疫反应的影响，我们使用了牛疱疹病毒1型（BHV-1）的主要糖蛋白之一糖蛋白D作为模型抗原。我们建立了一个小鼠模型，以评估在新型油包水佐剂VSA3中，用重组截短形式（tgD）或天然全长形式（gD）的BHV-1 gD进行免疫接种所引发的免疫反应。通过抗原特异性增殖反应和细胞因子（IFN-γ和IL-4）产生来评估，两种形式的BHV-1 gD抗原均诱导了良好水平的细胞介导免疫。初次免疫后，gD诱导的体液免疫反应优于tgD接种所引发的反应。然而，二次免疫后，两种形式的抗原均诱导了对BHV-1 gD强烈且相似的抗体反应。初次免疫后gD和tgD免疫原性的差异并非由于截短抗原中免疫原性表位的丧失或与佐剂VSA3结合的能力。我们的结果表明，gD和tgD均能够有效诱导细胞介导的免疫反应，并且尽管与全长gD相比，重组tgD在诱导初次体液免疫反应方面效率较低，但tgD能有效地引发二次抗体反应。
