Li Z, Hogan E L, Banik N L
Department of Neurology, Medical University of South Carolina, Charleston 29425, USA.
Neurochem Res. 1996 Apr;21(4):441-8. doi: 10.1007/BF02527708.
Impact spinal cord injury (20 g-cm) was induced in rat by weight drop. The immunoreactivity of mcalpain was examined in the lesion and adjacent areas of the cord following trauma. Increased calpain immunoreactivity was evident in the lesion compared to control and the immunostaining intensity progressively increased after injury. The calpain immunoreactivity was also increased increased in tissue adjacent to the lesion. mCalpain immunoreactivity was significantly stronger in glial and endothelial cells, motor neurons and nerve fibers in the lesion. The calpain immunoreactivity also increased in astrocytes and microglial cells in the adjacent areas. Proliferation of microglia and astrocytes identified by GSA histochemical staining and GFAP immunostaining, respectively, was seen at one and three days after injury. Many motor neurons in the ventral horn showed increased calpain immunoreactivity and were shrunken in the lesion. These studies indicate a pivotal role for calpain and the involvement of glial cells in the tissue destruction in spinal cord injury.
通过重物坠落对大鼠造成撞击性脊髓损伤（20克 - 厘米）。在创伤后，检测脊髓损伤部位及相邻区域mcalpain的免疫反应性。与对照组相比，损伤部位calpain免疫反应性明显增强，且损伤后免疫染色强度逐渐增加。损伤部位相邻组织中的calpain免疫反应性也增加。损伤部位的神经胶质细胞、内皮细胞、运动神经元和神经纤维中，mCalpain免疫反应性显著更强。相邻区域的星形胶质细胞和小胶质细胞中，calpain免疫反应性也增加。分别通过GSA组织化学染色和GFAP免疫染色鉴定发现，损伤后1天和3天小胶质细胞和星形胶质细胞出现增殖。腹角的许多运动神经元在损伤部位calpain免疫反应性增加且出现萎缩。这些研究表明calpain在脊髓损伤的组织破坏中起关键作用，且神经胶质细胞参与其中。
