Lee M Y, Kim C J, Shin S L, Moon S H, Chun M H
Department of Anatomy, Catholic University Medical College, Seoul, South Korea.
Neurosci Lett. 1998 Oct 16;255(2):79-82. doi: 10.1016/s0304-3940(98)00710-1.
Expression of ciliary neurotrophic factor (CNTF) was observed in reactive astrocytes in injured spinal cord of the adult rat. After unilateral incision of the dorsal funiculus at a midthoracic level, the rats were sacrificed on the day of postoperation (DPO) 3, 7, 14 or 28. Western blot analysis of spinal cord extracts showed that the intensity of the CNTF-immunoreactive band of 24 kDa was increased on DPO 3 and 7 and decreased on DPO 14. CNTF- or glial fibrillary acidic protein (GFAP)-like immunoreactivity was also increased progressively in the injured dorsal funiculus and adjacent gray matter. Double immunofluorescence histochemistry revealed that all CNTF-like immunoreactive cells showed GFAP-like immunoreactivity. The CNTF upregulation in the reactive astrocytes may play important roles in repair process after spinal cord injury.
在成年大鼠脊髓损伤后的反应性星形胶质细胞中观察到睫状神经营养因子（CNTF）的表达。在胸中段水平单侧切断背索后，于术后第3、7、14或28天处死大鼠。脊髓提取物的蛋白质免疫印迹分析显示，24 kDa的CNTF免疫反应条带强度在术后第3天和第7天增加，在术后第14天降低。在损伤的背索和相邻灰质中，CNTF或胶质纤维酸性蛋白（GFAP）样免疫反应性也逐渐增加。双重免疫荧光组织化学显示，所有CNTF样免疫反应性细胞均显示GFAP样免疫反应性。反应性星形胶质细胞中CNTF的上调可能在脊髓损伤后的修复过程中起重要作用。
