Wilson W R, Collins A C
Institute for Behavioral Genetics, University of Colorado, Boulder 80309, USA.
Alcohol. 1996 May-Jun;13(3):315-20. doi: 10.1016/0741-8329(95)02113-2.
The long-sleep (LS) and short-sleep (SS) lines of mice were selectively bred for differential sensitivity to the hypnotic effects of ethanol. Several studies suggest that excitatory amino acid receptor systems are involved in these genetically determined differences in sensitivity to ethanol. The experiments described in this article examine further the potential role of NMDA excitatory amino acid receptors in genetically determined differences in hypnotic sensitivity to ethanol by measuring [3H]MK-801 binding in eight brain regions of LS and SS lines of mice. Significantly greater levels of binding were found in SS hippocampus and striatum. Binding levels in the remaining brain regions revealed no significant between-line differences. Affinity differences between regions were seen but no between-line differences in affinity were found in any brain region. These findings lend support to the hypothesis that differences in NMDA receptor systems are part of the genetically determined biochemistry that produces differential hypnotic sensitivity to ethanol in LS and SS mice.
对小鼠的长睡眠（LS）和短睡眠（SS）品系进行了选择性培育，使其对乙醇的催眠作用具有不同的敏感性。多项研究表明，兴奋性氨基酸受体系统参与了这些对乙醇敏感性的基因决定差异。本文所述实验通过测量LS和SS品系小鼠八个脑区中的[3H]MK-801结合，进一步研究了NMDA兴奋性氨基酸受体在对乙醇催眠敏感性的基因决定差异中的潜在作用。在SS小鼠的海马体和纹状体中发现了显著更高的结合水平。其余脑区的结合水平在品系间未显示出显著差异。各脑区之间存在亲和力差异，但在任何脑区中均未发现品系间的亲和力差异。这些发现支持了以下假设：NMDA受体系统的差异是基因决定的生物化学的一部分，这种生物化学导致了LS和SS小鼠对乙醇产生不同的催眠敏感性。
