选定的唾液腺细胞培养以及异丙肾上腺素、血管活性肠肽和P物质的作用。

Selected salivary-gland cell culture and the effects of isoproterenol, vasoactive intestinal polypeptide and substance P.

作者信息

Horie K, Kagami H, Hiramatsu Y, Hata K, Shigetomi T, Ueda M

机构信息

Department of Oral Surgery, Nagoya University School of Medicine, Japan.

出版信息

Arch Oral Biol. 1996 Mar;41(3):243-52. doi: 10.1016/0003-9969(95)00132-8.

DOI:10.1016/0003-9969(95)00132-8

PMID:8735010

Abstract

To establish a selected salivary-gland cell culture and determine the effect of neuropeptides, monolayers were cultured using 3T3 cells as a feeder layer. To confirm the origin of these cultured cells, amylase production was examined by electron microscopy and periodic acid-Schiff staining, together with immunocytochemical analysis of myosin, anti-cytokeratin (CK-1, CK 10/13, CK MNF116, CK LMW, CK HMW and CK-19) and amylase antibody. The cultured cells demonstrated secretion granules containing amylase and presented features characteristic of acinar cells, which they retained until passage two. By using a feeder layer in conjunction with a newly formulated culture medium, the selectability of these cells was improved. Changes in proliferation of cultured salivary-gland cells in the presence of selected neurotransmitters were also examined. Isoproterenol enhanced cellular proliferation. On the other hand, vasoactive intestinal polypeptide and substance P, which increase the weight of salivary glands in vivo, showed no significant enhancement of proliferation.

摘要

为建立一种选定的唾液腺细胞培养体系并确定神经肽的作用，使用3T3细胞作为饲养层培养单层细胞。为确认这些培养细胞的来源，通过电子显微镜、过碘酸希夫染色以及对肌球蛋白、抗细胞角蛋白（CK-1、CK 10/13、CK MNF116、CK LMW、CK HMW和CK-19）和淀粉酶抗体的免疫细胞化学分析来检测淀粉酶的产生。培养的细胞显示出含有淀粉酶的分泌颗粒，并呈现出腺泡细胞的特征，这些特征一直保留到传代第二代。通过将饲养层与新配制的培养基结合使用，这些细胞的选择性得到了提高。还检测了在选定神经递质存在下培养的唾液腺细胞增殖的变化。异丙肾上腺素增强了细胞增殖。另一方面，在体内可增加唾液腺重量的血管活性肠肽和P物质，未显示出对增殖有明显增强作用。