Wang Y M, Wang J H, Pan F M, Tsai I H
Institute of Biological Chemistry, Academia Sinica, Taipei, Taiwan, Republic of China.
Toxicon. 1996 Apr;34(4):485-9. doi: 10.1016/0041-0101(95)00161-1.
The complete amino acid sequences of the Lys-49 PLA2s from the venom of Deinagkistrodon acutus (from Taiwan and China) and Trimeresurus mucrosquamatus (Taiwan habu) were solved by a facile cDNA cloning and sequencing method. The deduced amino acid sequences of the Lys-49 PLA2s of both venoms are identical, suggesting close phylogenic relationship between this two snake species of different genera. In addition, by cloning and cDNA sequencing, the mRNA coding for a Arg-49 PLA2 homolog of low expression level was also found in the venom gland of T. mucrosquamatus.
通过一种简便的cDNA克隆和测序方法，解析了来自尖吻蝮（来自台湾和中国大陆）及菜花原矛头蝮（台湾竹叶青）毒液中的Lys-49型磷脂酶A2（PLA2）的完整氨基酸序列。两种毒液的Lys-49型PLA2推导氨基酸序列相同，表明这两个不同属的蛇种之间存在密切的系统发育关系。此外，通过克隆和cDNA测序，在菜花原矛头蝮的毒腺中还发现了编码低表达水平的Arg-49型PLA2同源物的mRNA。
