McMahon J, McQuaid S
Neuropathology Laboratory, Royal Group of Hospitals, Belfast, Northern Ireland, UK.
Histochem J. 1996 Mar;28(3):157-64. doi: 10.1007/BF02331439.
Microwave irradiation was investigated as a pretreatment to in situ hybridization on formalin-fixed, paraffin-embedded tissue. Two probe/tissue systems were used: a single-stranded RNA probe for the detection of measles virus nucleocapsid genome in subacute sclerosing panencephalitis brain tissue, and a double stranded DNA probe for chicken anaemia virus in thymus of chicken infected with the virus. Microwaving, when used as sole pretreatment, was not as effective as the more traditional enzyme pretreatments for in situ hybridization. However, when used in combination with existing pretreatments, a significant increase was found in hybridization signal in both brain and thymus tissue. This was emphasized when combination enzyme/microwave pretreatments were used prior to detection of measles virus by in situ hybridization in a series of five archival subacute sclerosing panencephalitis cases. The use of microwave irradiation would be recommended as a means of supplementing in situ hybridization methods, especially when using long-term formalin fixed paraffin-embedded tissue.
研究了将微波辐射作为对福尔马林固定、石蜡包埋组织进行原位杂交的预处理方法。使用了两种探针/组织系统：一种单链RNA探针，用于检测亚急性硬化性全脑炎脑组织中的麻疹病毒核衣壳基因组；一种双链DNA探针，用于检测感染该病毒的鸡胸腺中的鸡贫血病毒。单独使用微波预处理时，对于原位杂交而言，其效果不如更传统的酶预处理方法。然而，当与现有的预处理方法联合使用时，在脑和胸腺组织中均发现杂交信号显著增加。在一系列五例存档的亚急性硬化性全脑炎病例中，通过原位杂交检测麻疹病毒之前使用酶/微波联合预处理时，这一点得到了强调。建议将微波辐射用作补充原位杂交方法的手段，尤其是在使用长期福尔马林固定石蜡包埋组织时。
