一项关于促性腺激素释放激素类似物在雄性大鼠中免疫原性的研究：与促性腺激素释放激素-半胱氨酸联合使用的各种佐剂的毒性比较。

An investigation into the immunogenicity of a GnRH analogue in male rats: a comparison of the toxicity of various adjuvants used in conjunction with GnRH-glycys.

作者信息

Ferro V A, O'Grady J E, Notman J, Stimson W H

机构信息

Department of Immunology, University of Strathclyde, Glasgow, UK.

出版信息

Vaccine. 1996 Apr;14(5):451-7. doi: 10.1016/0264-410x(95)00164-v.

DOI:10.1016/0264-410x(95)00164-v

PMID:8735559

Abstract

Immunization of male Copenhagen Fischer rats with a gonadotrophin releasing hormone (GnRH) analogue, conjugated to PPD resulted in high levels of antibody being produced which disrupted gonadal function in male rats. The antibody reduced serum testosterone levels and subsequently suppressed spermatogenesis. Alternatives to Freund's adjuvant were tested, namely, aluminium hydroxide and non-ionic surfactant vesicles (NISV). The study showed that aluminium hydroxide was as effective as Freund's adjuvant and less toxic, in both BCG and non-BCG primed animals. However, NISV were completely non-toxic and most effective in conjunction with BCG priming. The data obtained showed that NISV have the potential to be used as an alternative to FCA and aluminium hydroxide-based adjuvants.

摘要

用与纯化蛋白衍生物（PPD）偶联的促性腺激素释放激素（GnRH）类似物对雄性哥本哈根费希尔大鼠进行免疫，导致产生高水平抗体，从而破坏了雄性大鼠的性腺功能。该抗体降低了血清睾酮水平，进而抑制了精子发生。对弗氏佐剂的替代物进行了测试，即氢氧化铝和非离子表面活性剂囊泡（NISV）。研究表明，在卡介苗（BCG）和非BCG致敏动物中，氢氧化铝与弗氏佐剂一样有效且毒性较小。然而，NISV完全无毒，并且在与BCG致敏联合使用时最有效。所获得的数据表明，NISV有潜力用作弗氏完全佐剂（FCA）和氢氧化铝基佐剂的替代品。