Toupin J, Marks D H, Cooper E L, Lamoureux G
In Vitro. 1977 Apr;13(4):218-22. doi: 10.1007/BF02615078.
Contamination and low viability of earthworm coelemocytes in tissue culture have delayed in vitro studies. Using penicillin, streptomycin, tetracycline and Amphotericin B, Lumbricus terrestis coelomocytes were maintained viable and uncontaminated for 10 days at 15degreesC in medium L-15 supplemented with 5 to 10% fetal bovine serum. The coelomocytes survived for at least 10 days with 85% viability as assessed by trypan blue exclusion assays and phagocytosis of heat-killed yeast. Studies on the thymidine uptake, however, were negative. With the involvement of coelomocytes in tissue graft rejection, in vitro techniques can now be applied to study their capacity in the immune response.
蚯蚓体腔细胞在组织培养中的污染和低活力延缓了体外研究。使用青霉素、链霉素、四环素和两性霉素B，在添加了5%至10%胎牛血清的L-15培养基中，赤子爱胜蚓体腔细胞在15℃下可保持活力且未受污染10天。通过台盼蓝排斥试验和热灭活酵母吞噬作用评估，体腔细胞以85%的活力存活了至少10天。然而，关于胸腺嘧啶核苷摄取的研究结果为阴性。由于体腔细胞参与组织移植排斥反应，现在可以应用体外技术来研究它们在免疫反应中的能力。
