Kehrel B, Flicker E, Wigbels B, Osterfeld M, van de Loo J, Lüscher E F
University of Muenster, Department of Internal Medicine, Germany.
Blood Coagul Fibrinolysis. 1996 Mar;7(2):202-5. doi: 10.1097/00001721-199603000-00023.
Abnormal platelet activation may be involved in prethrombotic states and lead to thromboembolism. When platelets become activated, they release thrombospondin (TSP) from their alpha-granules which binds mainly to the surface of activated platelets, platelet-derived microparticles and other blood cells. To determine bound as well as free TSP in a single assay, we developed an indirect ELISA to measure TSP in fixed whole blood. The intra-assay variance was less than 5% and 97% of purified standard TSP, added to whole blood samples, was recovered with the ELISA. Blood collected with a 20G needle into a syringe resulted in lower "whole blood TSP' values than blood collected with the Vacutainer system. Whole blood TSP levels were measured in 66 healthy blood donors (20F, 46M) aged 25-75 years. The mean whole blood TSP concentration was 33 +/- 19 ng/ml. No significant difference in whole blood TSP was found between healthy females and males (35 +/- 23 ng/ml vs. 33 +/- 17 ng/ml).
血小板异常激活可能参与血栓前状态并导致血栓栓塞。当血小板被激活时，它们会从α颗粒中释放血小板反应蛋白（TSP），TSP主要与激活的血小板表面、血小板衍生的微粒及其他血细胞结合。为了在单次检测中同时测定结合型和游离型TSP，我们开发了一种间接ELISA法来检测固定全血中的TSP。该检测方法的批内变异小于5%，添加到全血样本中的97%的纯化标准TSP可通过ELISA法回收。用20G针头采血至注射器所得到的“全血TSP”值低于使用真空采血管系统采集的血样。对66名年龄在25至75岁之间的健康献血者（20名女性，46名男性）的全血TSP水平进行了测定。全血TSP浓度的平均值为33±19 ng/ml。健康女性和男性之间的全血TSP水平无显著差异（分别为35±23 ng/ml和33±17 ng/ml）。
