Kobayashi E, Motoki K, Yamaguchi Y, Uchida T, Fukushima H, Koezuka Y
Pharmaceutical Research Laboratory, Kirin Brewery Co., Ltd, Gunma, Japan.
Bioorg Med Chem. 1996 Apr;4(4):615-9. doi: 10.1016/0968-0896(96)00049-1.
We examined the in vitro and in vivo natural killer (NK) cell activity enhancing effects of alpha-, beta-galactosylceramide (GalCer) and alpha-, beta-glucosylceramide (GluCer) which have the same ceramide moiety, and of other alpha-, beta-GalCer having a different ceramide portion, and found that alpha-types show stronger enhancing effects than beta-types and the alpha-GalCer possesses the most potent activity among GalCers and GluCers having the same ceramide moiety. When the comparison of tumor growth inhibitory effects of alpha, beta-GalCer on mice subcutaneously inoculated with B16 cells was performed, the alpha-GalCer showed stronger suppressive activity than its beta-type, paralleling their enhancing effects on NK cell activity. These results suggest that the manner of combination between sugar and ceramide plays an important role in antitumor activity as well as enhancing effect on NK cell activity of GalCers.
我们研究了具有相同神经酰胺部分的α-、β-半乳糖基神经酰胺（GalCer）和α-、β-葡萄糖基神经酰胺（GluCer），以及具有不同神经酰胺部分的其他α-、β-GalCer对体外和体内自然杀伤（NK）细胞活性的增强作用，发现α型比β型表现出更强的增强作用，并且在具有相同神经酰胺部分的GalCers和GluCers中，α-GalCer具有最有效的活性。当对皮下接种B16细胞的小鼠进行α、β-GalCer的肿瘤生长抑制作用比较时，α-GalCer显示出比其β型更强的抑制活性，这与其对NK细胞活性的增强作用平行。这些结果表明，糖与神经酰胺的结合方式在GalCers的抗肿瘤活性以及对NK细胞活性的增强作用中起着重要作用。
