Lef J, Dege P, Scheucher M, Forsbach-Birk V, Clement J H, Knöchel W
Abteilung Biochemie, Universität Ulm, Germany.
Int J Dev Biol. 1996 Feb;40(1):245-53.
We have isolated and sequenced ten different members of the fork head/HNF-3 multigene family from Xenopus laevis which have been termed Xenopus fork head domain related (XFD) genes 1 to 10. Another four isolated genes (XFD' genes) represent pseudo-allelic variants which arose by an ancient tetraploidization within this species. Whereas all genes of this multigene family exhibit a high degree of sequence homology within the evolutionary conserved fork head domain, sequences outside this module are substantially different. Based upon sequence homologies over the entire coding sequences, XFD-7/7' represent the Xenopus homologs to the rodent hepatocyte nuclear factor HNF-3 alpha, while XFD-3/3' encode the homologs to HNF-3 beta. Here we present an analysis of the temporal transcription pattern of XFD genes 1 to 10 during embryogenesis and in some adult tissues. Eight of these XFD genes are activated during embryonic development, but show different and distinct transcription profiles. The localization of transcripts was determined by whole-mount in situ hybridization. Although transcription of individual XFD genes partially overlaps, each gene is characterized by means of a specific spatial pattern of transcriptional activity.
我们从非洲爪蟾中分离并测序了叉头/HNF-3多基因家族的10个不同成员，它们被命名为非洲爪蟾叉头结构域相关（XFD）基因1至10。另外4个分离出的基因（XFD'基因）代表了该物种内古老的四倍体化产生的假等位基因变体。虽然这个多基因家族的所有基因在进化保守的叉头结构域内都表现出高度的序列同源性，但该模块之外的序列则有很大差异。基于整个编码序列的序列同源性，XFD-7/7'代表啮齿动物肝细胞核因子HNF-3α在非洲爪蟾中的同源物，而XFD-3/3'编码HNF-3β的同源物。在此，我们展示了对XFD基因1至10在胚胎发育过程以及一些成体组织中的时间转录模式的分析。这些XFD基因中有8个在胚胎发育过程中被激活，但呈现出不同且独特的转录谱。转录本的定位通过整体原位杂交来确定。虽然单个XFD基因的转录部分重叠，但每个基因都具有特定的转录活性空间模式特征。
