Rudt F, Firmbach-Kraft I, Petersen M, Pieler T, Stick R
Georg-August-Universität Göttingen, Institut für Biochemie und Molekulare Zellbiologie, Germany.
Int J Dev Biol. 1996 Feb;40(1):273-8.
We use amphibian oocytes and eggs as favorite biological systems to study various cell biological phenomena. We have analyzed the role of the zinc finger protein TFIIIA and ribosomal protein L5 in nucleo-cytoplasmic transfer of 5S ribosomal RNA and report on the structural requirements of the 5S RNA for the interaction with TFIIIA. Furthermore, we have used the oocyte/egg system to analyze the kinetics of the posttranslational isoprenylation of oocyte nuclear lamin B3 and its fate during egg maturation. We demonstrate, that isoprenylation of newly synthesized lamins takes place in the oocyte cytoplasm before uptake into the nucleus and show, that the isoprene modifications alone are not sufficient to maintain stable association of lamins with nuclear envelope derived membranes in eggs. Finally, initial results of the identification of cis-acting sequence elements, involved in translational repression of lamin mRNAs in oocytes, are reported.
我们使用两栖类卵母细胞和卵子作为研究各种细胞生物学现象的理想生物系统。我们分析了锌指蛋白TFIIIA和核糖体蛋白L5在5S核糖体RNA核质转运中的作用，并报告了5S RNA与TFIIIA相互作用的结构要求。此外，我们利用卵母细胞/卵子系统分析了卵母细胞核纤层蛋白B3翻译后异戊二烯化的动力学及其在卵子成熟过程中的命运。我们证明，新合成的核纤层蛋白的异戊二烯化发生在卵母细胞细胞质中，在进入细胞核之前，并且表明仅异戊二烯修饰不足以维持核纤层蛋白与卵子中核膜衍生膜的稳定结合。最后，报告了鉴定参与卵母细胞中核纤层蛋白mRNA翻译抑制的顺式作用序列元件的初步结果。
