Prostran M, Varagić V M, Todorović Z, Jezdimirović M
Department of Pharmacology, Faculty of Medicine, Belgrade, Yugoslavia.
J Basic Clin Physiol Pharmacol. 1994 Apr-Jun;5(2):151-66. doi: 10.1515/jbcpp.1994.5.2.151.
The effects of physostigmine, L-arginine and NG-nitro-L-arginine methyl ester (L-NAME) were investigated in urethane-anaesthetized rats. The drugs were chosen because physostigmine has been known to produce an increase in peripheral adrenergic activity, whereas L-arginine and L-NAME have been known to modulate nitric oxide (NO) production. Slow infusion of L-arginine produced significant hypotension, but only in animals pretreated by physostigmine. L-NAME applied in the same way produced a slow developing increase in blood pressure, but not in animals pretreated by physostigmine. The pressor responses to physostigmine were potentiated if the drug was injected during infusion of L-NAME, and depressed if the drug was injected after stopping L-NAME infusion (in rats not pretreated with physostigmine). It is concluded that L-arginine-NO pathways act in vivo to oppose peripheral vasoconstrictor influences coupled with central cholinergically mediated activation of the adrenergic system, as produced by physostigmine. In this way, NO is part of a general mechanism for blood pressure regulation.
在乌拉坦麻醉的大鼠中研究了毒扁豆碱、L-精氨酸和N-硝基-L-精氨酸甲酯（L-NAME）的作用。选择这些药物是因为已知毒扁豆碱会使外周肾上腺素能活性增加，而L-精氨酸和L-NAME已知可调节一氧化氮（NO）的产生。缓慢输注L-精氨酸会产生显著的低血压，但仅在预先用毒扁豆碱处理的动物中出现。以同样方式应用的L-NAME会使血压缓慢升高，但在预先用毒扁豆碱处理的动物中则不会。如果在输注L-NAME期间注射毒扁豆碱，对毒扁豆碱的升压反应会增强；如果在停止L-NAME输注后注射毒扁豆碱（在未用毒扁豆碱预处理的大鼠中），则升压反应会减弱。得出的结论是，L-精氨酸-NO途径在体内发挥作用，以对抗与毒扁豆碱产生的中枢胆碱能介导的肾上腺素能系统激活相关的外周血管收缩影响。通过这种方式，NO是血压调节一般机制的一部分。
