Vnek J, Ikram H, Prince A M
Infect Immun. 1977 Apr;16(1):335-43. doi: 10.1128/iai.16.1.335-343.1977.
Hepatitis B surface antigen (HBsAg) was purified from approximately 8 liters of pooled plasma from a carrier chimpanzee. Precipitation of HBsAg with polyethylene glycol resulted in more than 20-fold purification, with about 80% recovery of antigenic activity. The sample was separated by further purification and fractionation into three populations of HBsAg-associated particles by column chromatography on hydroxylapatite: the first contained short filaments and 22- to 28-nm spheres, the second was composed of larger filaments and variable-sized spheres, and the third contained mostly 16- to 22-nm spherical particles. A large volume of the polyethylene glycol precipitate passed through hydroxyl-apatite twice yielded over 650 mg of partially purified HBsAg. A pooled preparation of purified HBsAg was separated by zone-convection electrofocusing into five peaks of antigenic activity within the pH range of 4.7 to 5.7.
乙肝表面抗原（HBsAg）从一只携带乙肝病毒的黑猩猩的约8升混合血浆中纯化而来。用聚乙二醇沉淀HBsAg可实现超过20倍的纯化，抗原活性回收率约为80%。通过在羟基磷灰石上进行柱色谱进一步纯化和分级分离，将样品分离为三种与HBsAg相关的颗粒群体：第一种包含短丝和22至28纳米的球体，第二种由较大的丝和大小不一的球体组成，第三种主要包含16至22纳米的球形颗粒。大量聚乙二醇沉淀物两次通过羟基磷灰石后，得到了超过650毫克的部分纯化HBsAg。通过区对流电聚焦将纯化HBsAg的合并制剂在4.7至5.7的pH范围内分离为五个抗原活性峰。
