Lauerma A I, Homey B, Vohr H W, Lee C H, Bloom E, Maibach H I
Department of Dermatology, University of California, San Francisco 94143-0989, USA.
Br J Dermatol. 1996 May;134(5):904-9.
Cis-urocanic acid (cUCA) has been suggested as a mediator of impairment of contact hypersensitivity induction by ultraviolet B (UVB) irradiation. We ascertained whether topical cUCA influences local lymph node activation during induction of contact hypersensitivity. Topical cUCA or vehicle was applied during the local lymph node assay to oxazolone. Local lymph node weight and cell number were assessed in all animals. Additionally, cell proliferation rate was studied in Hartley guinea-pigs and CBA/Ca mice, whereas activation of antigen-presenting cells was quantified in NMRI mice and Wistar rats. Topical cUCA suppressed all parameters of local lymph node activation due to oxazolone application in guinea-pigs. No effect, with the exception of a suppression of antigen-presenting cell activity, was seen in mice. No effect was seen in rats. The study shows that topical cUCA may suppress local lymph node activation during contact sensitization and suggests that differences between the effect of cUCA in different animal species may exist.
顺式尿刊酸（cUCA）被认为是紫外线B（UVB）照射导致接触性超敏反应诱导受损的一种介质。我们确定了局部应用cUCA是否会在接触性超敏反应诱导过程中影响局部淋巴结激活。在局部淋巴结试验期间，将局部应用的cUCA或赋形剂施加于恶唑酮。评估所有动物的局部淋巴结重量和细胞数量。此外，在Hartley豚鼠和CBA/Ca小鼠中研究了细胞增殖率，而在NMRI小鼠和Wistar大鼠中对抗抗原呈递细胞的激活进行了定量。局部应用cUCA抑制了豚鼠中因应用恶唑酮而导致的局部淋巴结激活的所有参数。在小鼠中，除了对抗抗原呈递细胞活性有抑制作用外，未观察到其他影响。在大鼠中未观察到影响。该研究表明，局部应用cUCA可能会在接触致敏过程中抑制局部淋巴结激活，并提示cUCA在不同动物物种中的作用可能存在差异。
