Abu-Roumi M, Newman M E, Yanai J
Melvin A. and Eleanor Ross Laboratory for Studies in Neural Birth Defects, Department of Anatomy and Embryology, Hebrew University-Hadassah Medical School, Jerusalem, Israel.
Brain Res Bull. 1996;40(3):183-6. doi: 10.1016/0361-9230(95)02131-0.
Mice were exposed to phenobarbital or heroin [diacetylmorphine (DAM)] prenatally by feeding the mother phenobarbital on gestation day 9-18; DAM was injected into the mother on gestation days 9-18. At the age of 50 days, mice exposed to phenobarbital or DAM prenatally were examined for long-term biochemical changes in the postsynaptic septohippocampal system as measured by alterations in formation of the second messenger inositol phosphate (i.p.). A significant increase in i.p. formation in response to carbachol was found after prenatal exposure to DAM. An increase in i.p. formation in response to 20 mM KCl alone or in the additional presence of 10 mM carbachol or 1mM physostigmine was found after prenatal exposure to phenobarbital or DAM. In addition, a significant increase in IP formation in response to sodium fluoride was found after prenatal exposure to phenobarbital or DAM. It is suggested that an increase in G-protein activation and in the second messenger formation accompanies the early drug-induced upregulation of the muscarinic receptors found in our previous studies.
在妊娠第9至18天给母鼠喂食苯巴比妥，使小鼠在产前接触苯巴比妥；在妊娠第9至18天给母鼠注射二乙酰吗啡（DAM，即海洛因）。在50日龄时，检测产前接触苯巴比妥或DAM的小鼠突触后隔海马系统的长期生化变化，通过测量第二信使肌醇磷酸（i.p.）形成的改变来进行检测。产前接触DAM后，发现对卡巴胆碱反应的i.p.形成显著增加。产前接触苯巴比妥或DAM后，发现单独对20 mM氯化钾或在额外存在10 mM卡巴胆碱或1 mM毒扁豆碱时的i.p.形成增加。此外，产前接触苯巴比妥或DAM后，发现对氟化钠反应的IP形成显著增加。提示在我们之前的研究中发现，早期药物诱导的毒蕈碱受体上调伴随着G蛋白激活和第二信使形成的增加。
