Mitsushima D, Mizuno T, Kimura F
Department of Physiology, Yokohama City University School of Medicine, Japan.
Neuroscience. 1996 May;72(2):429-34. doi: 10.1016/0306-4522(95)00572-2.
Extracellular levels of acetylcholine in the prefrontal cortex were measured using the micro-dialysis method in freely moving young (three to four months old) and old (23 to 24 months old) male rats over a period of 24 h to examine the effect of aging on prefrontal acetylcholine release. Prefrontal acetylcholine release during a 24 h period exhibited a diurnal variation with higher levels during the dark cycle than during the light cycle in young rats but not in old rats. In addition, prefrontal acetylcholine release was closely associated with spontaneous activity in young rats but not in old rats. The present study suggests that aging reduces diurnal changes in the prefrontal acetylcholine release and that there is a cross-correlation between the prefrontal acetylcholine release and spontaneous locomotor activity in male rats.
采用微透析法，在自由活动的年轻（3至4个月大）和老年（23至24个月大）雄性大鼠中，于24小时内测量前额叶皮质中的乙酰胆碱细胞外水平，以研究衰老对前额叶乙酰胆碱释放的影响。年轻大鼠在24小时内的前额叶乙酰胆碱释放呈现昼夜变化，黑暗周期中的水平高于光照周期，但老年大鼠并非如此。此外，年轻大鼠的前额叶乙酰胆碱释放与自发活动密切相关，而老年大鼠则不然。本研究表明，衰老会减少前额叶乙酰胆碱释放的昼夜变化，并且雄性大鼠的前额叶乙酰胆碱释放与自发运动活动之间存在相互关联。
