促性腺激素释放素对大鼠脊髓神经节神经元中γ-氨基丁酸诱导的去极化及γ-氨基丁酸激活电流的调节作用。
Modulatory effects of gonadorelin on GABA-induced depolarization and GABA-activated current in rat spinal ganglion neurons.
作者信息
Zhou X P, Wu X P, Guan B C, Li Z W
机构信息
Research Centre of Experimental Medicine, Tongji Medical University, Wuhan, China.
出版信息
Zhongguo Yao Li Xue Bao. 1996 Jan;17(1):31-4.
AIM
To explore the modulatory effects of gonadorelin on GABA-induced depolarization and GABA-activated current in membrane of rat primary sensory neurons.
METHODS
Intracellular recordings and whole-cell patch clamp techniques were performed on neurons in rat spinal ganglia (SG) preparation and neurons freshly isolated from rat SG, respectively. Drugs were applied by superfusion and/or by bath application.
RESULTS
In the majority of neurons GABA (10 mumol.L-1 -1 mmol.L-1) induced a depolarization, which was blocked by bicucullin (100 mumol.L-1). Pretreatment with gonadorelin (50 mumol.L-1) decreased the GABA-induced depolarization by 79 +/- 22% (n = 29), while gonadorelin elicited no effect or slight depolarization alone. In 6 of 11 cells, GABA-activated currents were also inhibited by pretreatment with gonadorelin (50 mumol.L-1), while in 5 of 11 cells, there was no change or a slight potentiation.
CONCLUSION
Gonadorelin exerts an inhibitory effect on GABA-induced depolarization and GABA-activated current in the primary sensory neurons.
目的
探讨促性腺激素释放激素对大鼠初级感觉神经元膜上γ-氨基丁酸（GABA）诱导的去极化及GABA激活电流的调节作用。
方法
分别采用细胞内记录和全细胞膜片钳技术，对大鼠脊髓神经节（SG）制备中的神经元和从大鼠SG新鲜分离的神经元进行实验。药物通过灌流和/或浴式给药。
结果
在大多数神经元中，GABA（10 μmol·L-1 - 1 mmol·L-1）诱导去极化，该去极化被荷包牡丹碱（100 μmol·L-1）阻断。用促性腺激素释放激素（50 μmol·L-1）预处理可使GABA诱导的去极化降低79±22%（n = 29），而促性腺激素释放激素单独作用时无效应或仅有轻微去极化。在11个细胞中的6个细胞中，用促性腺激素释放激素（50 μmol·L-1）预处理也可抑制GABA激活电流，而在11个细胞中的5个细胞中，无变化或有轻微增强。
结论
促性腺激素释放激素对初级感觉神经元中GABA诱导的去极化和GABA激活电流具有抑制作用。
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