Lloyd T L, Gupta S K, Gooding A E, Alianti J R
Glaxo Research Institute, Research Triangle Park, NC 27709, USA.
J Chromatogr B Biomed Appl. 1996 Apr 12;678(2):261-7. doi: 10.1016/0378-4347(95)00491-2.
A method of analysis for the determination of alosetron in human plasma or serum has been developed. The method was fully automated using a laboratory robot in order to improve analytical precision, efficiency and safety. The assay involved solid-phase extraction with reversed-phase HPLC separation and fluorescence detection. A validation exercise over the concentration range of 0.1 to 20 ng/ml demonstrated the selectivity, linearity, sensitivity, accuracy, precision, extraction efficiency, ruggedness and stability of the method. The method has been applied in support of numerous human pharmacokinetic/biopharmaceutic studies over the last five years.
