Tomioka Y, Suzuki H, Miura K, Yamaguchi T, Hishinuma T, Mizugaki M
Department of Pharmaceutical Sciences, Tohoku University Hospital, Miyagi, Japan.
Methods Find Exp Clin Pharmacol. 1996 Apr;18(3):189-95.
A flow cytometric study was performed on the liver of normal and clofibrate-treated rats. The flow cytometric patterns of these fractions showed three distinct populations (R1, R2 and R3). The R3 region was remarkably increased in the clofibrate-treated nuclear fraction, and was applied to sucrose linear density gradient centrifugation. Mt1, Mt2, Mt3 and Mt4 fractions were isolated and reacted with rhodamine-123. An essential enzyme for beta-oxidation, delta 3, delta 2-enoyl-CoA isomerase, was mainly expressed in Mt1 of the control-nuclear fraction, but not in Mt1, Mt2 and Mt4 in the clofibrate-treated nuclear fraction. These results suggest that clofibrate affects the flow cytometrical population of the mitochondria and changes the expression level of beta-oxidation enzyme(s) of the mitochondria in rat liver.
对正常大鼠和经氯贝丁酯处理的大鼠的肝脏进行了流式细胞术研究。这些组分的流式细胞术图谱显示出三个不同的群体（R1、R2和R3）。在经氯贝丁酯处理的核组分中，R3区域显著增加，并应用于蔗糖线性密度梯度离心。分离出Mt1、Mt2、Mt3和Mt4组分，并与罗丹明-123反应。β-氧化的一种关键酶，δ3，δ2-烯酰辅酶A异构酶，主要在对照核组分的Mt1中表达，但在经氯贝丁酯处理的核组分的Mt1、Mt2和Mt4中不表达。这些结果表明，氯贝丁酯影响线粒体的流式细胞群体，并改变大鼠肝脏中线粒体β-氧化酶的表达水平。
