Ito Y, Sakagami H, Kondo H
Department of Anatomy, Tohoku University School of Medicine, Sendai, Japan.
Brain Res Mol Brain Res. 1996 Apr;37(1-2):329-32. doi: 10.1016/0169-328x(95)00329-q.
We attempted to see whether or not gene expression of phosphatidylinositol 3-kinase (PI 3-kinase) is changeable in the hypoglossal nucleus of rats after hypoglossal nerve crushing by in situ hybridization histochemistry. After unilateral nerve crushing, an apparent enhancement of gene expression for PI 3-kinase was observed in individual neurons of the hypoglossal nucleus at the operated side on the first postoperative day, and it was sustained for 7 postoperative days. Thereafter the expression decreased at the operated side and no significant difference in the expression level was noticed between the operated nucleus and the contralateral, non-operated or sham-operated nucleus on postoperative day 14. The present study suggests that PI 3-kinase contributes to some important roles in morphological changes of mature neurons associated with nerve regeneration.
我们试图通过原位杂交组织化学法，观察大鼠舌下神经切断后，舌下神经核中磷脂酰肌醇3激酶（PI 3激酶）的基因表达是否会发生变化。单侧神经切断后，在术后第1天，手术侧舌下神经核的单个神经元中观察到PI 3激酶基因表达明显增强，并持续7天。此后，手术侧的表达下降，术后第14天，手术侧核与对侧未手术或假手术的核之间在表达水平上没有显著差异。本研究表明，PI 3激酶在与神经再生相关的成熟神经元形态变化中发挥着重要作用。
