Smith D, Bradley S J, Scott G M
Department of Haematology, University College London Hospitals, UK.
J Hosp Infect. 1996 May;33(1):71-6. doi: 10.1016/s0195-6701(96)90031-6.
As part of an audit of the processing of autologous bone marrow, we found that marrow was often contaminated with organisms potentially pathogenic to neutropenic recipients. One of 14 marrows studied was found to be contaminated before the processing stage and five others became contaminated during processing. The organisms isolated at these stages were Propionibacterium sp., coagulase-negative staphylococci, Staphylococcus aureus and coryneforms, suggesting that the skin was the likely source of contamination. Five out of the 11 marrows returned to patients were found to be contaminated after thawing. Two of these were marrows previously shown to be contaminated with coagulase-negative staphylococci before freezing, and from these coagulase-negative staphylococci were isolated again, in one case the strains were indistinguishable. New organisms isolated after thawing included Bacillus sp. and Corynebacterium sporogenes suggesting contamination from the environment. No infections attributable to these organisms were demonstrated in any of the patients studied.
作为自体骨髓处理审计的一部分，我们发现骨髓常被可能对中性粒细胞减少的接受者致病的生物体污染。在研究的14份骨髓中，有1份在处理阶段之前就被发现受到污染，另外5份在处理过程中被污染。在这些阶段分离出的生物体有丙酸杆菌属、凝固酶阴性葡萄球菌、金黄色葡萄球菌和棒状杆菌，这表明皮肤可能是污染来源。在回输给患者的11份骨髓中，有5份在解冻后被发现受到污染。其中2份是之前显示在冷冻前被凝固酶阴性葡萄球菌污染的骨髓，并且从这些骨髓中再次分离出凝固酶阴性葡萄球菌，在一个案例中菌株无法区分。解冻后分离出的新生物体包括芽孢杆菌属和产芽孢棒状杆菌，提示来自环境的污染。在任何研究的患者中均未证实有归因于这些生物体的感染。
