Effects of administration of N-methyl-D,L-aspartate (NMA) on gonadotropin secretion in untreated and steroid-treated ovariectomized mares during the breeding season and in intact and ovariectomized mares during anestrus.

The purpose of this experiment was to investigate whether N-methyl-D,L-aspartate stimulated gonadotropin secretion in mares and to determine the response in two experimental paradigms where gonadotropin secretion is low or elevated. In Experiment 1, conducted during the breeding season (summer), eight long-term ovariectomized mares were treated daily for 21 d with progesterone plus estradiol (n = 4) or oil vehicle. Beginning on Day 14, each mare received, in a randomized design on alternate days, an intravenous injection of either 0, 0.5, 1.0, or 5.0 mg/kg NMA. Treatment with NMA was not accompanied by a change in gonadotropin release in vehicle-treated ovariectomized mares. In steroid-treated mares, gonadotropin values were suppressed compared with vehicle controls, but NMA only induced a significant increase in follicle-stimulating hormone (FSH) and only at the highest dose. In Experiment 2, the effect of the administration of NMA was compared in intact anestrous mares (n = 4) and long-term ovariectomized mares (n = 4) during the nonbreeding season. At the highest doses (1.0 and 5.0 mg/kg), NMA induced a significant increase in luteinizing hormone (LH) and FSH in intact mares. In contrast, pretreatment concentrations of LH and FSH were higher in long-term ovariectomized mares and NMA only increased LH, but not FSH, at the highest dose. The results demonstrate that the administration of NMA stimulates the release of LH and FSH in mares. During anestrus, ovarian intact mares appear more responsive than long-term ovariectomized mares. At high doses, NMA may induce differential release of the gonadotropins in long-term ovariectomized mares.