Kandoussi A M, Hugue V, Parra H J, Dracon M, Fruchart J C, Tacquet A, Cachera C
Laboratoire de Recherches Néphrologiques, Hôpital Calmette, Lille, France.
Am J Nephrol. 1996;16(4):287-92. doi: 10.1159/000169011.
Whole plasma from 6 normolipidemic chronic renal failure (CRF) patients undergoing hemodialysis treatment was passed through the anti-apolipoprotein (Apo) AI immunosorbent column connected to the anti-Apo B immunoaffinity column. Apo AI and B containing particles were analyzed for lipid and Apo contents. The results were compared with findings obtained in age-matched normolipidemic healthy controls. Although plasma Apo AI and AII levels decreased in CRF patients, the concentrations of Apo CII, CIII, and E coeluted with Apo AI were similar to those of the controls. The slightly elevated plasma concentrations of Apo CII and CIII in the CRF patients studied were shown to be associated with Apo B containing particles. The nonretained fraction from both groups contains no Apo AI and no Apo B, but still contains lipids and other Apo such as Apo AII and Apo CII. The occurrence of approximately 29% of plasma Apo E in this fraction constitutes the main abnormality found in these patients (< 5% in controls). A two-phase electroimmunoassay shows that this Apo E did not correspond to the plasma E-AII complex. These findings show that the compositional alterations of Apo AI and Apo B containing particles in CRF patients were observed even in normolipidemic patients and suggest that the kidney may play a metabolic role in the removal of free forms of lipoprotein particles such as free Apo E.
将6名接受血液透析治疗的血脂正常的慢性肾衰竭（CRF）患者的全血通过连接抗载脂蛋白（Apo）B免疫亲和柱的抗Apo AI免疫吸附柱。对含有Apo AI和B的颗粒进行脂质和Apo含量分析。将结果与年龄匹配的血脂正常的健康对照者的结果进行比较。尽管CRF患者血浆Apo AI和AII水平降低，但与Apo AI共洗脱的Apo CII、CIII和E的浓度与对照组相似。研究的CRF患者中血浆Apo CII和CIII浓度略有升高，表明与含Apo B的颗粒有关。两组的未保留部分不含Apo AI和Apo B，但仍含有脂质和其他Apo，如Apo AII和Apo CII。该部分中约29%的血浆Apo E的出现是这些患者中发现的主要异常情况（对照组中<5%）。双相免疫电泳显示，这种Apo E与血浆E-AII复合物不符。这些发现表明，即使在血脂正常的患者中也观察到CRF患者含Apo AI和Apo B颗粒的组成改变，提示肾脏可能在清除游离形式的脂蛋白颗粒如游离Apo E中起代谢作用。
