Wirth C, Schwuchow J, Jonas L
Department of Pathology, University of Rostock, Germany.
Acta Histochem. 1996 Apr;98(2):165-72. doi: 10.1016/S0065-1281(96)80035-3.
The uptake of the lectin wheat germ agglutinin (WGA) by the rat pancreas was studied after intraperitoneal injection of FITC-labelled WGA. Strong fluorescence was seen in the interstitial space of the pancreatic parenchyma and on the basolateral surface of acinar cells after one h. Two or four h after injection there was also a fluorescence inside the acinar cells. After intraparenchymal injection of colloidal gold-labelled WGA into the pancreatic gland in situ or in vitro incubation of isolated rat pancreatic acinar cells with WGA-gold, an internalization of the lectin by receptor mediated endocytosis was observed electron microscopically. Clathrin-coated pits or vesicles could not be observed. The results suggest a direct uptake of WGA from the peritoneum into the gland by diffusion and its binding as well as internalization by the pancreatic acinar cells.
腹腔注射异硫氰酸荧光素标记的麦胚凝集素（WGA）后，对大鼠胰腺摄取该凝集素的情况进行了研究。1小时后，在胰腺实质的间质空间以及腺泡细胞的基底外侧表面可见强荧光。注射后2或4小时，腺泡细胞内也出现荧光。将胶体金标记的WGA原位注射到胰腺实质内，或使分离的大鼠胰腺腺泡细胞与WGA-金进行体外孵育后，通过电子显微镜观察到凝集素通过受体介导的内吞作用发生内化。未观察到网格蛋白包被的小窝或小泡。结果表明，WGA通过扩散从腹膜直接摄取到腺体中，并被胰腺腺泡细胞结合以及内化。
